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. Author manuscript; available in PMC: 2015 Apr 6.
Published in final edited form as: Toxicology. 2014 Feb 19;318:59–68. doi: 10.1016/j.tox.2014.01.010

Fig. 4.

Fig. 4

DZ and DZO increase ROS production in hippocampal neurons. Hippocampal neurons were plated in Neurobasal/FBS (10%) medium for 2 h before being washed and switched to ACM for an additional 46 h. Neurons were probed with 10 μM H2DCF-DA for 30 min, after which the probe was washed out and neurons were treated with DZ or DZO for 1 h. H2 O2 (20 μM) was used as a positive control. Results are expressed as mean (±SEM) of at least three independent experiments in which treatments were performed in triplicate. Significantly different from control, *p < 0.05, ***p < 0.001.