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. 2014 Feb 4;60(2):128–135. doi: 10.1262/jrd.2013-098

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©2014 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 3. — Relative mRNA expression of maternal genes (Bmp15, Gdf9, cyclin B1, and c-mos) (A) and poly(A) tail length (B) in FCCP- or ddC-treated MII oocytes. Porcine Gapdh mRNA expression was used as an internal control, and the control group was set at 1. Ctrl, control; F, FCCP; D, ddC. Lane 1, control; lanes 2 and 3, oocytes treated with 200 or 2000 nM FCCP, respectively; lanes 4 and 5, oocytes treated with 200 or 2000 µM ddC, respectively. Arrows point to the initial length of the poly(A) tail. Values represent means ± SEM from three separate experiments. *P < 0.05, compared with the control.