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. 2014 Feb 17;60(2):83–91. doi: 10.1262/jrd.2013-080

Table 2. Primers for real-time PCR analysis.

Gene Primer sequence (5’ to 3’) Product length (bp)
GATA-4-F
GATA-4-R
acaaccaccgaggaaatgcg
ggagactggctgatggctgac
188
GATA-6-F
GATA-6-R
ccaggaagcgaaaacctaagaac
cctgaagctgacggttgtgtg
154
β-actin-F
β-actin-R
caacgagcggttcaggtgt
tggagttgaaggtggtctcg
92
18S-F
18S-R
ttggtggagcgatttgtc
atctcgggtggctgaacg
129

F, forward primer; R, reverse primer; β-actin and ribosomal 18S rRNAs were selected as the reference genes.