Table 2. Primers for real-time PCR analysis.
Gene | Primer sequence (5’ to 3’) | Product length (bp) |
GATA-4-F GATA-4-R |
acaaccaccgaggaaatgcg ggagactggctgatggctgac |
188 |
GATA-6-F GATA-6-R |
ccaggaagcgaaaacctaagaac cctgaagctgacggttgtgtg |
154 |
β-actin-F β-actin-R |
caacgagcggttcaggtgt tggagttgaaggtggtctcg |
92 |
18S-F 18S-R |
ttggtggagcgatttgtc atctcgggtggctgaacg |
129 |
F, forward primer; R, reverse primer; β-actin and ribosomal 18S rRNAs were selected as the reference genes.