List of used antibodies.
| Factor | Subunit | Gene | Weight, cDNA / (size, a.a.r.) |
Function within NER | Interaction with other factors |
|---|---|---|---|---|---|
| XPC | HR23B | hhr23b | 43 / (409) | Recognition of a distorted DNA structure | TFIIH XPA DDB |
| XPC | xpc | 125 / (940) | |||
| CEN 2 | cen2 | 20 / (172) | |||
| DDB | DDB1 | ddb1 | 127 / (1140) | Recognition of damage, interaction with chromatin | XPC RPA |
| DDB1 | ddb2 | 48 / (428) | |||
| XPA | XPA | xpa | 31 / (273) | Structural function, binding to a damaged strand | XPA RPA TFIIH ERCC 1 |
| RPA | RPA70 | rpa1 | 68 / (616) | Binding to single-stranded DNA | XPA XPG PCN A/RFC |
| RPA32 | rpa2 | 30 / (270) | |||
| RPA14 | rpa3 | 14 / (121) | |||
| TFIIH | XPB | xpb | 89 / (782) | ATPase, minor helicase activity 3'→5'- DNA-helicase | XPA XPC XPF XPG |
| XPD | xpd | 87 / (760) | ATP-dependent 5'→3'-DNA-helicase; testing of modification presence | ||
| p62 | gtf2h1 | 62 / (548) | Core subunit, stimulates XPB | ||
| p44 | gtf2h2 | gtf2h2 44 / (395) | Core subunit, stimulates XPD | ||
| p34 | gtf2h3 | 34 / (308) | DNA binding | ||
| p52 | gtf2h4 | 52 / (462) | Regulatory subunit for ATPase activity of XPB functioning in TFIIH complex | ||
| p8 | gtf2h5 (ttda) |
8 / (71) | Interaction with P52, stimulation of ATPase activity of XPB | ||
| Mat1 | mnat1 | 36 / (309) | Member of the CAK complex | ||
| Cdk7 | cdk7 | 39 / (346) | Phosphorylates RN A-polymerase II and other substrates | ||
| Cyclin | ccnh | 38 / (323) | Regulation of cell cycle | ||
| XPF | ERCC1 | ercc1 | 33 / (297) | Endonuclease, catalyzes formation of single-strand break in DNA on the 5’ side of the damage |
XPA TFIIH |
| XPF | xpf | 103 / (905) | |||
| XPG | XPG | xpg | 133 / (1186) | Endonuclease, catalyzes formation of single-strand break in DNA on the 3’ side of the damage |
TFIIH RPA PCN A |
| RFC | RFC1 | rfc1 | 128 / (1148) | ATP-dependent connection of PCN A | PCN A RPA |
| RFC2 | rfc2 | 39 / (354) | |||
| RFC3 | rfc3 | 41 / (356) | |||
| RFC4 | rfc4 | 40 / (363) | |||
| RFC5 | rfc5 | 38 / (340) | |||
| PCNA | PCNA | pcna | 3X37 / (3X261) | Factor ensuring processivity of DNA polymerases | RFC XPG Polδ |
| Polδ | p125 | p125 | 124 / (1107) | DNA polymerase | PCNA |
| p66 | p66 | 51 / (466) | |||
| p50 | p50 | 51 / (469) | |||
| p12 | p12 | 12 / (107) | |||
| Polε | p261 | p261 | 261 / (2286) | DNA polymerase | PCNA |
| p59 | p59 | 60 / (527) | |||
| p17 | p17 | 17 / (147) | |||
| p12 | p12 | 12 / (117) | |||
| Ligase I | Ligase I | ligI | 102 / (919) | Ligation of a single-strand break | |
| Ligase III | Ligase III | ligIII | 103 / (862) |