Fig. 1.

Generation of human iDP cells. Morphology of cultured DP cells before reprogramming factors infection (A) and emergence of first reprogrammed clones on the 8^th day after infection (B) and 11^th day after infection (C). Expression of alkaline phosphatase in nonreprogrammed (passage 6) DP cells (D) and in generated (passage 6) iDP cells (E). Immunofluorescent detection of nucleus proteins: Oct4(F), Sox2 (G), and Nanog (H), and surface antigens: SSEA3 (L), SSEA4(M), Tra- 1 -60 (N), and Tra -1-81 (O) in iDP cells. G, I, K, L-O - nuclei are counterstained with DAPI (blue). Scale bars = 200 μm. (G, I, K – the same visual fiels as F, H, J respectively)