Fig. 2.

Ratio of untreated (DP) to reprogrammed (iDP) cells expressing pluripotency markers, Tra-1 -60 , Tra-1-81 , SSEA3, and SSEA4, according to flow cytofluorometry (A). Normal diploid karyotype (46,XX) of iDP cells. Chromosomes are stained with DAPI (green) and 7-AAD (red) (B). Telomerase reactivation in iDP cells (C). Telomerase is active in the samples, with additional bands corresponding to telomeric repeats (iDP, ESCs). Control: ES and iDP cells heated before telomerase inactivation (iDP) (inact). Telomerase of the initial iDP cell culture is inactive. Reverse transcription- PCR (RT-PCR) analysis of iDP cells (D). Expression levels of Oct4, Sox2, Nanog, Tert, Klf4, Lin28, Dppa4, Dnmt3 in DP, iDP, and ES cells (E), according to real-time PCR