Fig. 3.

iDP cells differentiation in vitro. Formation and characterization of embryoid bodies (EBs) in iDP cell cultures (A–D), and in the control, human ES cells (E–H). Morphology of EBs in iDP (A) and ES (E) cell cultures. Expression of markers of three germinal layers, nestin (Nes), desmin (Des), and alpha-fetoprotein (AFP), EBs differentiation in iDP- (B–D) and hESderived cells (F–H) EBs. Directed differentiation of iDP cells (I–T). Expression of osteogenic differentiation markers: osteonectin (ON, I) and osteopontin (OP, J). Expression of hepatocyte differentiation markers: Foxa2 (K), Hnf4α (L), cytokeratin18 (CK18, M), alphafetoprotein (AFP, N), and albumin (ALB, O). Expression of neuronal differentiation markers Prox1 (P), nestin (Nes, Q) doublecortin (DC, R), neuron-specific enolase (NSE, S), and β-III- tubulin (Tub3b, T). Scale bars = 200 μm