List of used antibodies.
| Antibodies (antigens) | Manufacturer | Antibody producer | Working dilution |
|---|---|---|---|
| Oct4 | Millipore (MAB4401) | Mouse | 1 : 200 |
| Sox2 | Cell Signalling (#3579) | Rabbit | 1 : 250 |
| Nanog | Abcam (ab80892) | Rabbit | 1 : 400 |
| SSEA3 | STEMCELL Technologies (#01553) | Rat | 1 : 100 |
| SSEA4 | Abcam (ab16286) | Mouse | 1 : 250 |
| Tra-1-60 | Abcam (ab16288) | Mouse | 1 : 100 |
| Tra-1-81 | STEMCELL Technologies(#01556) | Mouse | 1 : 300 |
| Desmin | Abcam (ab15200) | Rabbit | 1 : 200 |
| Nestin | Millipore (MAB5326) | Mouse | 1 : 200 |
| Doublecortin | Abcam (ab77450) | Rabbit | 1 : 200 |
| β-III-tubulin | Millipore (MAB1637) | Mouse | 1 : 100 |
| Neuron-specific enolase | DakoCytomation (M087329) | Mouse | 1 : 200 |
| Vimentin | Abcam (ab8978) | Mouse | 1 : 200 |
| Pan-cytokeratin | Santa-Cruz (sc-81714) | Mouse | 1 : 20 - 1 : 50 |
| Osteopontin | Millipore (MAB3057) | Rat | 1 : 350 |
| Osteonectin | Millipore (AB1858) | Rabbit | 1 : 500 |
| Hnf4α | Santa-Cruz (sc-8987) | Rabbit | 1 : 50 |
| Foxa2 | Millipore (#07-633) | Mouse | 1 : 100 |
| Alpha-fetoprotein | R & D Systems (MAB1368) | Mouse | 1 : 200 |
| Albumin | R & D Systems (MAB1455) | Mouse | 1 : 250 |
| CK8 | Millipore (04-588) | Rabbit | 1 : 200 |
| CK18 | Millipore (MAB3234) | Mouse | 1 : 100 |
| Alexa Fluor 488 | Molecular Probes (# A-11029, A-21206, A-21208) | Goat, donkey | 1 : 500 |
| Alexa Fluor 594 | Molecular Probes (# A-11032) | Goat | 1 : 500 |