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. 2014 Apr 25;4:4798. doi: 10.1038/srep04798

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(a) Thermotactic behavior test. Thermotactic behavior of larvae was tested on a 2.5% agar plate with temperature gradient between 21°C and 28°C (shown on the right). Typically, ~50 larvae were aligned along the medial line and the numbers of animals moved in the warm and cool halves were counted after 3 min. Response index (RI) was calculated as indicated. (b) Thermotactic response indices of Tdc2-dTrpA1 larvae. While the w (CS10) control larvae preferred cooler temperatures exhibiting negative thermotactic RI, all the larvae carrying the Tdc2-dTrpA1 construct preferred warm temperatures exhibiting positive thermotactic RI. ***p < 0.001 by Mann-Whitney U-test with w (CS10). Number of sample is indicated in the bar.