RNA was analyzed immediately after the cells were isolated (0 hour) and after 12,
24, and 48 hours in culture. The relative gene expression levels for CTBs isolated
from placentas of patients who delivered due to nPTL (n = 5) or
sPE (n = 5) are shown as a heat map, ranging from high (red) to
low (blue). The sPE CTBs were from the following cases (tiled from left to right):
(a) HELLP syndrome and IUGR; (b) sPE; (c) sPE and IUGR; (d) superimposed sPE; and
(e) HELLP syndrome. One sample of nPTL CTBs collected at 48 hours was omitted for
technical reasons. The fold changes for each time point (sPE vs. nPTL) are shown
on the right. ns, no significant difference (LIMMA); t, no significant difference
in expression (sPE vs. nPTL) by 48 hours (maSigPro).