Fig.1.

Testin interacts with Vangl2 in the yeast two-hybrid assay.

(A) Schematic diagram of the two hybrid screen in which the C-terminal cytoplasmic tail of Vangl2, Vangl2^C, was fused in-frame with Gal4 DNA binding domain (gal4-BD) and a cochlear cDNA was fused to Gal4 DNA activation domain (Gal4-AD). (B) Yeast cells were serially diluted and replicated on rich media YPDA (yeast media containing yeast extract, peptone, dextrose plus adenine) plates and minimal media plates lacking Leucine (L), Tryptophan (W), Histidine (H), Adenosine (Ade). The survival of cells on the minimum media depends on the presence of the two plasmids (Gal4-BD and Gal4-AD) and the expression of reporter genes. pGBTK7 plasmid contains Gal4-DB only while pVangl2-C contains Vangl2^C fused to Gal4-BD.