Figure 6.

Vimentin-deficient platelets have a reduced platelet adhesion to collagen and VWF. Whole blood from WT (n = 6) or vimentin knockout mice (n = 7) was perfused over surfaces coated with (A) collagen fibrils or (B) purified plasma murine VWF at a shear rate of 1500 seconds⁻¹. After perfusion, the plates were washed with buffer, and the attached platelets were recorded and quantified as described in “Methods.” The bar graph in A shows the percent of surface covered by the adhered platelets in 20 different fields of view (mean ± SD, *P < .05,), whereas B shows the average number of adhered platelets per view field as described in Figure 3 (**P = .10).