Table 3.

Transcripts of important immune receptors underrepresented in MCs

	MC ex vivo*			MC cultured†			Cultured + stimulated‡
Gene	D2	D3	D4	D1	D8	D5	D1	D8	D5	First primary sample§	Mean all||
Receptors involved in antigen presentation
HLA-DRA	45	30	25	0.1	0	0	0	0	0.1	26 120 (migratory LC)	798
HLA-DPA1	28	20	16	8.8	22	18	10	27	16	13 278 (migratory LC)	408
HLA-DPB1	16	13	17	2	4.6	7.5	0.9	13	4.2	13 319 (migratory LC)	239
HLA-DQA1	0.5	1	6.2	0	0.4	0	0	0	0	17 246 (migratory LC)	143
HLA-DQA2	26	1.4	4	0	0	0	0	0	0.1	3 963 (migratory LC)	38
Chemokine receptors
CCR1	0.2	0	0	0	0	0	0	0.7	0	705 (stim. monocyte)	18
CCR2	0	0.4	0.2	0	1.2	0.1	0.3	0.7	0	178 (monocyte)	5.3
CCR3	0	0	0	0	0	0	0	0	0	190 (whole blood)	2.3
CCR4	2.6	0.5	1.4	21	6.6	80	1.3	3.5	27	1 384 (memory Treg)	19
CCR5	0	0	0.2	0.4	2.5	0.7	0	0.7	0.8	199 (stim. monocyte)	7.8
CCRL2	0	0	0.2	0.4	0.2	1.2	3.4	0.7	1	470 (stim. monocyte)	8
CXCR3	0.7	0.4	0.5	4.2	0.2	5.6	4.1	7.7	40	359 (plasmacytoid DC)	5.9
IL8RA/CXCR1	0	0	0	0	0	0	0	0	0	1 216 (whole blood)	13
Pattern recognition receptors
TLR1	0.9	1.2	1.2	0.3	1.9	0.4	0	0.7	2.4	434 (neutrophil)	12
TLR2	4.7	3.9	2.3	0	1	0	0	0	0.4	896 (eosinophil)	29
TLR3	1.4	0.7	0.5	3.9	2.5	3.4	0.3	0.7	3.6	114 (placenta)	6.5
TLR4	3.5	11	5.4	75	22	44	28	16	28	1 085 (stim. monocyte)	55
TLR7	0.9	0.4	0.8	0.1	0.2	0	0	0	1.5	343 (plasmacytoid DC)	7.7
TLR9	0	0	0	0	0	0	0	0	0	162 (plasmacytoid DC)	1.9

DC, dendritic cell; LC, Langerhans cell; stim., stimulated; Treg, regulatory T cell.

^*Donor 2/donor 3/donor 4 (directly upon isolation from skin).

^†Donor 1/donor 8/donor 5 (MCs expanded in culture for 4-5 weeks).

^‡Donor 1 stimulated for 2.5 hours/donor 8 stimulated for 5 hours/donor 5 stimulated for 16 hours.

^§Nontransformed cell/tissue sample with (second) highest expression of the gene.

^||Mean expression of all non-MC FANTOM5 samples. The values are given in (rounded) tags per million tags; if <10, 1 decimal place is given.