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. 2014 Mar 19;14:49. doi: 10.1186/1471-230X-14-49

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Copyright © 2014 Fukuda and Fujita; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The Discriminant Score (Ds) for group I to group IV was calculated by using all OTU values. The Ds value was seen to decrease in the following order group I > group IIa > group IIb > group III > group IV, but the variation was large and the tendency was still uncertain. Group comparison was made by using One Way ANOVA, group I vs group IV (P < 0.01); group I vs group IIb (P < 0.05); group IIa vs group IV (P < 0.01); group III vs group IV (P < 0.05).