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. 2014 Mar 20;15:219. doi: 10.1186/1471-2164-15-219

Figure 21.

Figure 21

Validation of microarray expression of 12 selected genes by qRT-PCR. A total of 12 genes were selected in the validation using qRT-PCR: (A)Phenylalanine ammonia-lyase (PAL: isotig 10873), (B)Cinnamate-4-hydroxylase (C4H: isotig 09462), (C)4-Coumarate:CoA ligase (4CL: isotig 04988), (D)Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: isotig 10178), (E)p-Coumarate-3-hydroxylase (C3H: isotig 02271), (F)Caffeoyl-CoA O-methyltransferase; Cinnamyl alcohol dehydrogenase (CCoAOMT: isotig 10196), (G)Cinnamoyl-CoA reductase (CCR: isotig 05079), (H)Cinnamyl alcohol dehydrogenase (CAD: isotig 02638), (I)Sucrose synthase (Sus: isotig 12351), (J)Cellulose synthase (Ces: isotig 08498), (K) α-Tubulin (Tub α: isotig 02753), (L)β−Tubulin (Tub β: isotig 13384). In qRT-PCR, Ubiquitin (UBQ) was used as a reference gene, and the data were calibrated relative to transcript levels in the 24 March sample. Error bars show standard deviation for three replicates.