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. 2014 Mar 19;14:67. doi: 10.1186/1471-2229-14-67

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Copyright © 2014 Estevan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Arabidopsis TEV-susceptibility protein AteIFiso4E does not interact with TEV VPg in yeast two-hybrid assays. Yeast transformed with both bait (fused to the Gal4 binding domain, BD) and prey (fused to the Gal4 activation domain, AD) vectors were spotted on selective dropout medium without leucine and tryptophan (SD-LW) as a control and on selective dropout without leucine, tryptophan and histidine (SD-LWH) to check for interaction between both partners. In each case, a control with an empty vector (-) was included to confirm there was no self-activation. All combinations are shown in triplicate.