Figure 4.

Homologous complementation of eifiso4e rtm1 by AteIFiso4E overexpression restores susceptibility to TuMV and TEV HAT. A, Schematic representations of the T-DNA constructs inserted in eifiso4e rtm1 Arabidopsis plants. B, One-month-old Arabidopsis plants were inoculated with the TuMV CDN1 strain and assayed for viral coat protein accumulation by DAS-ELISA at 24 dpi. One T2 line transformed with the 35S:GUS construct was tested and five independent T2 lines transformed with 35S:AteIFiso4E. C, Western blot analysis of eIFiso4E protein levels in total proteins extracted from 1-month-old leaves. Actin protein levels were assessed as a loading control. D, One-month-old Arabidopsis plants were inoculated with TEV-HAT and assayed for viral coat protein accumulation by ELISA at 24 dpi.