Figure 5.

Heterologous complementation of eifiso4e rtm1 with Capsicum annuum eIF4E1 alleles trigger susceptibility to TEV HAT in an allele-specific manner. A, Schematic representations of the T-DNA constructs inserted in eifiso4e rtm1 Arabidopsis plants. B, One- month-old Arabidopsis plants were inoculated with TEV HAT and assayed for viral coat protein accumulation by ELISA at 24 dpi. Results are shown on three independent T2 lines per construct. C, RT-PCR on total mRNA extracted from 1-month-old plants show that the Ca.eIF4E mRNA is expressed at similar levels in eifiso4e rtm1 plants transformed with T-DNA harbouring a 35S:Ca.eIF4E-pvr2⁺ or 35S:Ca.eIF4E1-pvr2² construct. The reference gene APT1 is amplified as a control.