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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Environ Mol Mutagen. 2013 Nov 9;55(3):184–195. doi: 10.1002/em.21823

Fig. 2.

Fig. 2

Graphic representation of Sequenom Epityper assays with transcription factor binding sites. A. The CES2 assay examines 4 individual CpG sites located approximately 1,600 to 1,900 bp upstream of the TSS. The CES2 CpG site #3 is found within an ETS transcription factor-binding site. B. The COMT assay covers 30 CpGs sites, although due to technical limitations (e.g. small fragment sizes are not detectable) Sequenom Epi-Typer outputs only 17 CpG units, spanning approximately 250 bp upstream and 100 bp downstream of the TSS. An ETS transcription factor-binding site coincides with CpG sites #30, and #31 on COMT. C. Although CpG methylation on the ETS consensus sequence of SULT2A1 could not be directly assessed (CpG site #1), the assay investigates six individual CpG sites (four CpG units) surrounding the target site, spanning approximately 1,650 to 1,950 bp upstream of the TSS. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]