Figure 6.
mRNA expression of: (a) AP, (b) collagen type I (Col I), and (c) osteocalcin (OC) from CTPs after 9 and 30 days on CA postmicrotextures and smooth CA surfaces. The real-time RT-PCR was repeated four times, and the data are normalized to corresponding control surfaces (set to 1.0) within a particular experiment to account for differences in CTPs among individual donors. AP mRNA expressed slightly higher on day 9 compared to day 30 on smooth surfaces. In contrast, the mRNA expression of AP has increased by day 30 on postmicrotextures. On day 9, Col I mRNA expression on both postmicrotextures and smooth surfaces is similar. The mRNA expression of Col I was increased on postmicrotextures on day 30, but there was similar expression of Col I mRNA on smooth surfaces. Even though OC mRNA expression was almost the same on day 9 for both smooth and posttextured surfaces, it expressed more strongly on postmicrotextures on day 30. Numerical values denote mean and standard errors. * denotes statistical significance (p < 0.05).