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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Science. 2008 Jul 31;321(5895):1496–1499. doi: 10.1126/science.1158712

Figure 2. DB stalk cells proliferate and differentiate into tracheoblasts.

Figure 2

(A–C) Fate mapping a DB4 stalk cell (clone O, Table I). (A) Schematic showing labeled cell. (B) DB4 cell labeled with cytoplasmic GFP. All tracheal nuclei express DsRed (btl-Gal4; UAS-nuclearDsRed). (C) Same DB three days later. Labeled DB4 generated 7-cell clone. DB2 and contralateral DB2 (DB2') are indicated along with degenerating contralateral DB1 (arrowhead), which was also labeled in L2 larva. (D–F) DB4 cells at three different stages of mitosis in L3 larvae immunostained for phospho-histone H3 (red) and beta-galactosidase (trh-lacZ, green). Tubular DB4 cell divides along short axis of tube (F, F’). Fig. S6 shows low magnification. (G–J) Individual DB stalk cells in Tr4 and Tr7, and nearby epidermal cells of newly molted trh-lacZ L3 larva labeled with BrdU during L1 and L2 then co-stained after molting for BrdU (red) and beta-galactosidase (green). Fig. S7 shows low magnification. Bars, 25 µm, except G-J,10 µm.