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. 2014 Mar 19;15:211. doi: 10.1186/1471-2164-15-211

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Copyright © 2014 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Subcellular localization of selected BnaCPK-GFP fusion proteins in intact N. benthamiana leaf epidermal cells and protoplasts. Panels A-E represent the subcellular localizations of BnaCPK5-GFP, BnaCPK9-GFP, BnaCPK11-GFP, BnaCPK13-GFP and BnaCPK15-GFP, together with a plasma marker AtCBL1n fused with mCherry, respectively. A white arrow marks the region and direction in which the distribution of fluorescence intensities was scanned. The left panel is GFP fluorescence, the middle mCherry field and the right represents an overlay of the two images. PM, plasma membrane; nu, nuclei. Bar = 50 μm.