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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biochem Pharmacol. 2013 Jun 14;86(5):584–596. doi: 10.1016/j.bcp.2013.06.004

Fig. 2.

Fig. 2

Induction of apoptosis by Fas-agonistic antibody (CH-11), TNF-α and TRAIL in PgpON/OFF HeLa cells. Elevating concentrations of Fas-agonistic antibody (CH-11) (A), TNF-α (B) in the presence of CHX (500 ng/ml) and TRAIL (C and D) were added to PgpOFF (white) and PgpON (gray) HeLa cells for 24 h. Apoptotic cell death was measured using Annexin V and PI staining, as described in Section 2. No Annexin V+/PI− (early apoptotic) cells were observed after 24 h incubation with these death ligands. TRAIL effect was tested in the absence (C) or presence (D) of CHX. Results are mean ± SD values of triplicate samples from one representative experiment of 3 independent experiments (*P < 0.05, **P < 0.01).