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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Biochem Pharmacol. 2013 Jun 14;86(5):584–596. doi: 10.1016/j.bcp.2013.06.004

Fig. 9.

Fig. 9

Analysis of the role of TRAIL-receptors in TRAIL-induced apoptosis of PgpON/OFF HeLa cells. (A) HeLa PgpOFF cells were pre-incubated with various concentrations of monoclonal neutralizing antibodies to TRAIL receptors DR4 (white) and DR5 (gray) 1 h prior to treatment with 15 ng/ml TRAIL for 24 h. In the control experiment, cells were pre-treated with mouse IgG1. Specific lysis was determined using PI staining. (B)PgpOFF (white) and PgpON (gray) HeLa cells were pretreated with the neutralizing antibodies anti-DR4, anti-DR5 or both (5 μg/ml each) 1 h before treatment with 15 ng/ml TRAIL for 24 h. Specific lysis was then determined using PI staining. In the control experiment, cells were pre-treated with mouse IgG1. (C) Percentage of neutralization of lysis by anti-DR4 and anti-DR5 was calculated as 100 × (1 − (%lysed cells in sample)/(%lysed cells in control). HeLa PgpOFF cells (white) cells and HeLa PgpON cells (gray) are depicted. Results are mean ± SD values of triplicate samples from one representative experiment of 3 independent experiments (*P < 0.05, **P < 0.01).