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. 2014 Mar 21;13:44. doi: 10.1186/1475-2859-13-44

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Copyright © 2014 Zhou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression, purification and DAL activity assay of the fusion protein. A. Expression of the soluble fusion protein for eDAL or sDAL with a specified tag followed by tevS in E. coli. The fusion proteins from the supernatants were detected by Western blot using anti-His6 antibody. The lane 1–6 represented His6MBP-tevS-eDAL, His6MBP-tevS-sDAL, His6GST-tevS-eDAL, His6GST-tevS-sDAL, His6-tevS-eDAL and His6-tevS-sDAL. B. Purification of the fusion proteins. The fusion proteins were purified by Ni-NTA and analyzed by SDS-PAGE. All the proteins are indicated by arrows. C. The activity of the DAL as the fusion partner in the crude extracts. D. The activity of the purified fusion proteins for DAL.