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. 2014 Mar 21;13:44. doi: 10.1186/1475-2859-13-44

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Copyright © 2014 Zhou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression, purification and activity assay of eDAL with a His6GST followed by the specified protease cleavage site. A. SDS-PAGE analysis of the soluble fusion proteins expressed in E. coli. U: uninduced; I: induced. B. SDS-PAGE analysis of the purified fusion proteins. In the Figure 2A and 2B, all the proteins are indicated by arrows. C. DAL activity of the fusion protein in the crude extracts. D. DAL activity of the purified fusion protein.