TABLE 1.
Primer name | Sequence | Direction |
---|---|---|
Ehmeth C228S 5′ | GATAAAAGGACTTCATGTTTTACTAAGTCA | Sense |
Ehmeth C228S 3′ | TGACTTAGTAAAACATGAAGTCCTTTTATC | Antisense |
Ehmeth C229S 5′ | AAAAGGACTTGTTCATTTACTAAGTCATAT | Sense |
Ehmeth C229S 3′ | ATATGACTTAGTAAATGAACAAGTCCTTTT | Antisense |
Ehmeth C228S-C229S 5′ | GATAAAAGGACTTCATCATTTACTAAGTCA | Sense |
Ehmeth C228S-C229S 3′ | TGACTTAGTAAATGATGAAGTCCTTTTATC | Antisense |
rDNA 5′ | ATGGTGAACAATCATACCTT | Sense |
rDNA 3′ | TTATCGGATGTGTGAGCCC | Antisense |
Universal primer | CGCGCGAAGCTTAATACGACTCACTATA | |
tRNAAsp primer | TGGCGCTTCAACGGGGATT |