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Mixed infection by Toxoplasma and Neospora reveals that c-Myc is actively induced by Toxoplasma. HFFs were infected at an MOI of 1 with a mixture of Toxoplasma and Neospora tachyzoites. c-Myc levels were examined by IFA 20 hpi using anti-c-Myc antibodies. Toxoplasma and Neospora parasites were differentiated using antibodies to the Toxoplasma surface marker SAG1. Toxo, Toxoplasma gondii (red triangles); Neo, Neospora caninum (blue triangles).
