FIG 6.

JNK inhibitor treatment abolishes induction of c-Myc in Toxoplasma-infected cells. HFFs were treated with 25 μM JNK inhibitor or with DMSO for 30 min prior to infection. Cells were infected at an MOI of 1 with Toxoplasma RH parasites or mock infected, and cell lysates were harvested 5 hpi. FBS-stimulated cells pretreated with either JNK inhibitor or DMSO served as a control for JNK inhibition. Whole-cell lysates were subjected to Western blot analysis using anti-c-Myc and anti-GAPDH antibodies. Anti-SAG1 was used to ensure that similar levels of infection were achieved in all samples.