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. 2014 Jan 20;18(4):624–634. doi: 10.1111/jcmm.12210

Figure 1.

Figure 1

B27 supplement is required to maintain standard cardiospheres (CSs) culture yield and gene expression profile. Primary and secondary CSs yield and dimension (A) were altered in B27− CGM, expressed as percentage of effect, normalized to B27+ CGM (n = 3). Cell proliferation, quantified by WST colorimetric assay, of CS-derived cells (CDCs) from both B27+/− was comparable (B). Representative images of B27+/− CSs are shown in C. Gene expression analysis by realtime PCR for multiple markers of stemness and cardiovascular differentiation (D) revealed no differences in the profile of B27+/− CDCs, while a dramatic drop in c-kit levels in B27− IICSs was detectable (n = 3 each). The marked size of error bars for c-kit and TnI in (D) is due to the fact that in one replicate, expression levels were undetectable. #P < 0.05 versus B27+. *P < 0.001 versus B27+. Scale bars = 100 μm.