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Autologous serum is not able to support cardiospheres (CSs) formation after primary explant. Representative images of explant cultures show rapid initial growth in autologous human serum (aHS) compared with foetal bovine serum (A), and higher cell yield (n = 5) in terms of harvest of CS-forming cells (B). Once plated to form CSs, though, cells in aHS stopped proliferating (C), as demonstrated by WST assay (n = 5). #P < 0.05. Scale bars = 50 μm.
