Figure 4.

Cardiospheres (CSs) yield and cell proliferation in AB human sera cultures. CSs yield and dimension, expressed as percentage of effect versus foetal bovine serum (FBS), were comparable in human serum (HSs) cultures (A), until CS-forming cells could be harvested. Representative images of CSs from different sera are shown in B. Nevertheless, at the CS-derived cell (CDC) stage cells stopped proliferating compared with control cultures in FBS (C), and such effect was dose dependent, as demonstrated by comparing cultures in 3% and 20% sera (D). Representative images of CDCs in HSs and FBS are shown in E. Scale bars = 50 μm. *P < 0.001 versus HSs.