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. 2014 Jan 20;18(4):624–634. doi: 10.1111/jcmm.12210

Figure 5.

Figure 5

Cardiac progenitor cells in AB human sera displayed altered commitment towards cardiovascular lineages. Gene expression analysis on CS-derived cells (CDCs; A), normalized to standard foetal bovine serum (FBS) conditions, revealed a significant up-regulation of KDR in human sera (HSs), and a significant down-regulation of SMA and Thy1, suggesting a preferred commitment towards an endothelial lineage. Immunofluorescence and confocal analysis of primary cardiospheres (B) further evidenced a higher expression of the endothelial marker CD31 in HSs, particularly in Starfish samples. CDCs obtained from standard FBS were cultured for 1 week in HS 5% or 20%, subjected or not to heat inactivation (HI). Gene expression analysis (C) revealed that 1 week of HS exposure was enough to significantly down-regulate multiple genes versus FBS, such as SMA, c-kit, TnI, Cx43, Thy1 and GATA4. As observed for cell proliferation, the effect was dose dependent. Heat inactivation partially recovered the effect, but important genes, such as SMA, c-kit and Cx43, were still significantly down-regulated compared with standard FBS conditions. Scale bars = 50 μm. *P < 0.001 versus FBS.