Table 3.
Antibodies for Western blot
| Antibody | Host | Vendor | Dilution | Molecular weight (kDa) |
|---|---|---|---|---|
| iNOS/NOS2 |
Rabbit |
BD Biosciences |
1:4000 |
130 |
| α-Actinin |
Mouse |
BD Biosciences |
1:5000 |
105 |
| p- NFκB p65 (Ser536) |
Rabbit |
Cell Signaling |
1:1000 |
65 |
| NFκB p65 |
Rabbit |
Cell Signaling |
1:1000 |
65 |
| p-PKR (Thr451) |
Rabbit |
Sigma-Aldrich |
1:500 |
65 |
| GAPDH |
Mouse |
Millipore |
1:2000 |
36 |
| p-eIF2α (Ser51) |
Rabbit |
Abcam |
1:500 |
36 |
| α-rabbit-HRP conjugated |
Goat |
Jackson ImmunoResearch |
1:5000 |
|
| α-mouse-HRP conjugated | Goat | Jackson ImmunoResearch | 1:2000 |