Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 25;460(Pt 1):127–139. doi: 10.1042/BJ20140334

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

(A) MALDI–TOF spectra of non-phospho-ubiquitin (top panel) and phospho-Ser⁶⁵ ubiquitin (bottom panel) after incubation with MBP–PINK1 after separation on a Mono Q column. (B) MALDI–TOF spectra of non-phosphorylated Ubl (Parkin residues 1–76) (top panel) and mixed Ubl species (~60% phosphorylated and ~40% non-phosphorylated) (bottom panel). (A and B) In total, 2 μg of the non-phospho-ubiquitin and phospho-ubiquitin (A) or non-phospho-Ubl and phospho-Ser⁶⁵ Ubl (B) were resolved by SDS/PAGE, followed by staining with Colloidal Coomassie Blue for quality control.