Table 3. Steady state kinetic constants for LcA reactions utilizing various SNAP25 substrates.
| SNAP25 Substrate | Km (µM)a | kcat (Sec−1)a | kcat/Km (µM/Sec) |
| 17-merb | 1513±281.8 | 28±3.5 | 0.019 |
| 66-mer | 32.43±0.9281 | 23±0.22 | 0.72 |
| Full Length | 33.34±4.592 | 76±2.2 | 2.3 |
a
The values are averages of results from 5, 10, and 15-min reactions reported in Table 2.
b
10 min incubation data. Kinetic parameters were obtained by hyperbolic Michaelis-Menten curve fitting (R2 = 0.99345) of experimental 5 replicate data.