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. 2014 Mar 25;27(5):145–155. doi: 10.1093/protein/gzu007

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© The Author 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Characterisation of yeast SUMO-binding Adhirons, Ad-ySUMO 10, 15, 20 and 22. (A) Biotinylated Ad-ySUMOs were used to detect ySUMO (black). Human SUMO 1 (light grey) and human SUMO 2 (dark grey) by ELISA with TMB product detected at 560 nm. (B) Western blots using biotinylated Ad-ySUMO clones against yeast SUMO alone (upper panel) and mixed with 20 µg of HEK293 cell lysate (lower panel). (C) Western blot analysis using biotinylated Ad-ySUMO clones against yeast and human SUMOs 1 and 2. (D) Isothermal calorimetry of Ad-ySUMOs binding to yeast SUMO with the isotherms and the data fits.