Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Am J Reprod Immunol. 2013 Nov 29;71(1):86–93. doi: 10.1111/aji.12179

Figure 2. MMP-9 activity by zymography and ELISA.

Figure 2

2A. Zimography. The culture media of choriodecidual and intervillous placental blood leukocytes (n=7) collected at 0, 24, 48, and 72 h were analyzed in gelatin-substrate gels. The electrophoretic positions of the gelatinolytic activities (clear bands) show the 92 kDa inactive enzyme and the 82 kDa active form of MMP-9, respectively. AM=culture medium of U937 cells as activity marker. 2B.ELISA. Active and total MMP-9 in the same media was quantitated. Each point represents the mean ± SD (n=3).