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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Nature. 2014 Feb 23;508(7494):88–92. doi: 10.1038/nature13028

Extended Data Figure 8. Object recognition memory and preference for novelty is preserved in CA2-TeNT animals.

Extended Data Figure 8

a, Schema of the experimental design for the novel object recognition task. b, The groups did not differ significantly in exploration of object 1 (YFP, 16.75 ± 1.57s; TeNT, 19.60 ± 2.24s) or object 2 (YFP, 16.50 ± 1.97s; TeNT, 15.90 ± 1.66s) averaged over the course of the first 4 trials (two-way ANOVA: Treatment × Object F(1,32) = 0.80, P = 0.38; Object F(1,32) = 1.05, P = 0.31; Treatment F(1,32) = 0.34, P = 0.56; YFP, n = 8; TeNT, n = 10). c, Both groups explored the novel object (YFP, 21.23 ± 2.37s; TeNT, 24.37 ± 2.81s) more than the familiar object (YFP, 7.41 ± 0.92s; TeNT, 8.57 ± 1.48s). Statistical analysis revealed a significant effect of object, but not CA2 inactivation or interaction of the two (two-way ANOVA: Treatment × Object F(1,28) = 0.22, P = 0.64; Object F(1,28) = 48.46, P < 0.0001; Treatment F(1,28) = 1.02, P = 0.32). Multiple comparison testing revealed a significant difference between exploration of the novel object compared to exploration of the old object for both the YFP group (P = 0.0002) and the TeNT group (P < 0.0001). d, Schema of the experimental design for another variation of the novel object recognition task. e, The groups did not differ significantly in time spent exploring object 1 (YFP, 21.50 ± 2.31s; TeNT, 22.18 ± 3.57s) or object 2 (YFP, 22.02 ± 2.23s; TeNT, 22.36 ± 2.81s) during trial 1 of day 4 (two-way ANOVA: Treatment × Object F(1,44) = 0.004, P = 0.95; Object F(1,44) = 0.02, P = 0.90; Treatment F(1,44) = 0.03, P = 0.85; YFP, n = 12; TeNT, n = 12). f, Both groups explored the novel object (YFP, 21.49 ± 1.91s; TeNT, 22.73 ± 1.82s) more than the familiar object (YFP, 13.74 ± 1.83s; TeNT, 16.53 ± 1.64s). Statistical analysis revealed a significant effect of object, but not CA2 inactivation or interaction of the two (two-way ANOVA: Treatment × Object F(1,44) = 0.18, P = 0.67; Object F(1,44) = 15.02, P = 0.0004; Treatment F(1,44) = 1.25, P = 0.27). Multiple comparison testing revealed a significant difference between exploration of the novel object compared to exploration of the old object for both the YFP group (P = 0.008) and the TeNT group (P = 0.02). Results are presented as mean ± s.e.m.