Skip to main content
. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Nat Commun. 2014 Apr 3;5:3509. doi: 10.1038/ncomms4509

Figure 1. RIP mutation strongly depends on homology length.

Figure 1

a, RIP is quantified by the number of mutations in a random sample of germinated spores. RIP substrates are introduced into haploid nuclei of Strain A (filled circles, orange). Maternal homokaryotic tissues originate from wild-type Strain B (filled circles, magenta). RIP occurs in heterokaryotic cells (outlined in red) containing nuclei of both parental strains. The heterokaryotic cell and the nuclei are not drawn to scale.

b, Direct perfect repeats of graded length assayed for RIP in c, d, e. The total sequenced region and the longest sub-region that is invariant among all constructs are indicated.

c, The total number of mutated DNA strands for repeat constructs in b. 48 strands (24 spores from a single cross) were analyzed for each construct (Table 1).

d, The total number of mutations in the invariant region (green) and its two sub-regions: 802-bp (blue, some or all of which is part of the repeat unit) and the 706-bp (grey, linker). Regression analysis is based only on mutation counts over the whole invariant region and includes the medium (524-220 bp) repeat range. r represents Pearson's correlation coefficient.

e, Mutation profiles of selected repeat constructs.