Figure 6. RIP mutation is proportional to the number of triplet frames and does not require MEI3 (RAD51).

a, (i-iv) For each indicated Test segment (red, green, or blue), all potential frames for aligning Test and Ref segments by triplets with the matching periodicity of 11 bp are given. The total number of such frames and overall homology between Test and Ref segments are indicated. Lines (i) and (ii) (red): two partial homologies analyzed in Fig. 3c, d. Line (iii) (green): a Test segment that contains as much overall homology as (i) and as many triplet frames as (ii). Line (iv) (blue): a Test segment that provides no triplet frames, but contains more overall homology than (ii) and nearly as much overall homology as (i).

b, The mean number of mutations over the total sequenced region for constructs in Table 1 (blue, red, and green) plotted against the amount of overall homology (left panel) or the number of triplet frames as defined in a (right panel). r represents Pearson's correlation coefficient. Correlation coefficients for the mean and the median values were computed separately.

c, Strategy for examining the role of MEI3 in RIP (Methods). Strains SR0 and SR1 were produced from a cross between strains #12433 (Δmei-3) and #12440 (Δspo11) which, along with reference wild-type strain #2489 were obtained from the Fungal Genetics Stock Center²⁴.

d, Strains used in c were phenotyped and genotyped by PCR. PCR bands correspond to wild-type alleles or the Dp::BAR duplication. The Δmei-3 genotype is verified by the histidine sensitivity assay.

e, Mutation profiles summarize 10 spores analyzed from each cross. f, The mean number of mutations (per spore) over the total sequenced region for the crosses shown in e. Mutation counts were compared by the two-sided K-S test: NS P ≥ 0.05.

Error bars represent s.e.m.