Figure 7. RIP mutation depends on global disposition of interacting repeats.

a, Analyzed constructs contain one or more of the following elements positioned, as shown, to either side of a 707-bp or a 303-bp linker region (grey): 337 bp of perfect homology (turquoise) in direct or inverted orientation; 500 bp of partial homology 4H-7N (blue and blue/white) in direct or inverted orientation, either alone or adjacent to 337 bp of perfect homology in inverted orientation, and with or without 22 bp of random sequence inserted between the perfect and partial homology segments.

b, The number of mutations within the partial homology repeat (left panel) and the perfect homology repeat (right panel) in the invariant region. For (ii-vii), corresponding mutation profiles shown in d. *** P < 0.001; ** P < 0.01, NS P ≥ 0.05, ns P-value fluctuates around 0.05. Error bars represent s.e.m.

c, Mutation density profiles for constructs (v) and (vii) reveal the effect of the 22-bp insertion at junction between perfect and imperfect inverted repeat units. The invariant region (1000 bp) was divided into sequential non-overlapping 50-bp bins. The number of mutations for each 50-bp bin is plotted. Mutations from two replica crosses for each construct were analyzed together.

d, Mutation profiles for repeat constructs ii-vii. Each profile is based on 24 spores (ii, iii, v, vi and vii) or 30 spores (iv) from one representative cross.