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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Nat Commun. 2014 Apr 3;5:3509. doi: 10.1038/ncomms4509

Table 1.

Repeat constructs analyzed in this study

Construct %H #F Plasmid Strain “A” Replica crosses P value Sample size Mean SEM Median MAD
802 bp 100 N/A pEAG115A T103.3 1 N/A 24 296.2 14.2 304.5 48.5
524 bp 100 N/A pEAG115B T193.3 1 N/A 24 123.2 7.2 117.0 10.0
460 bp 100 N/A pEAG115D T194.2 1 N/A 24 70.8 7.0 72.5 20.5
400 bp 100 N/A pEAG115E T196.5 1 N/A 24 35.5 4.6 35.5 17.0
337 bp 100 N/A pEAG115C T187.3 1 N/A 24 18.6 2.9 17.0 9.5
279 bp 100 N/A pEAG115F T198.3 1 N/A 24 9.0 1.9 6.0 5.0
220 bp 100 N/A pEAG115G T199.5 1 N/A 24 2.5 1.0 1.0 1.0
155 bp 100 N/A pEAG115H T200.3 2 1.00 48 0.1 0.1 0.0 0.0
Ref 100 N/A pEAG186A T207.3 1 N/A 30 68.6 4.8 72.5 17.5
A[19] N/A N/A pEAG186C T209.1 1 N/A 30 64.2 4.0 62.5 13.5
N[19] N/A N/A pEAG186D T210.11 1 N/A 30 79.6 4.8 83.0 23.5
CA[11] N/A N/A pEAG186Q T251.1 1 N/A 30 74.7 6.5 74.5 32.0
9H-2N 82 7 pEAG186E T211.1 1 N/A 30 57.3 5.4 56.5 21.5
8H-3N 73 6 pEAG186G T213.1 1 N/A 30 46.2 3.8 45.5 20.5
7H-4N 64 5 pEAG186H T214.5 1 N/A 30 44.3 3.5 42.0 13.0
6H-5N 55 4 pEAG186I T215.13 2 0.17 60 42.8 3.0 37.5 11.5
5H-6N 45 3 pEAG186J T216.7 2 0.38 60 27.7 2.4 25.0 12.5
4H-7N 36 2 pEAG186K T221.18 2 0.67 60 24.1 2.3 21.0 12.5
4H-7N′ 36 2 pEAG200D T254.4 2 0.81 60 20.7 2.4 16.0 11.0
3H-8N 27 1 pEAG186L T223.4 3 0.14 90 14.0 1.5 9.5 6.0
3H-8N′ 27 1 pEAG200L T279.12 2 0.10 60 10.2 1.3 8.0 6.0
3H-9N 25 1 pEAG186S T272.3 2 0.16 60 12.1 1.9 6.0 6.0
3H-1N-3H-4N 55 2 pEAG186M T222.6 2 0.61 60 24.9 2.2 23.5 12.5
3H-2N-3H-3N 55 2 pEAG200X T334.8 2 0.32 60 28.6 3.2 23.5 18.0
2H-2N 50 0 pEAG186W T248.1 3 0.64 90 6.0 1.2 2.0 2.0
5H-4N 56 0 pEAG200T T296.1 2 0.97 60 6.9 1.6 1.5 1.5
5H-7N 42 0 pEAG200P T294.3 2 0.18 60 5.7 1.6 1.0 1.0
5H-8N 38 0 pEAG200Q T295.5 2 0.56 60 6.1 1.7 1.0 1.0
3H-7N 30 0 pEAG186R T258.2 2 0.20 60 3.7 0.7 1.0 1.0
3H-10N 23 0 pEAG200N T281.2 2 0.88 60 1.1 0.3 0.0 0.0
GFP 20 0 pEAG186B T208.3 2 0.42 60 2.8 0.9 0.0 0.0
2H-9N 18 0 pEAG186T T246.1 3 0.39 90 3.8 0.9 1.0 1.0
0H-11N 0 0 pEAG186Z T259.1 3 0.44 90 1.5 0.4 0.0 0.0
5H-17N 23 0 pEAG186O T230.4 2 0.48 60 9.2 2.0 5.5 5.0
7H-15N 32 0 pEAG186N T261.1 2 0.18 60 13.0 1.8 8.5 7.5
337[D] N/A N/A pEAG115CX T202.4 1 N/A 24 34.3 3.5 31.5 11.5
337[I] N/A N/A pEAG115CY T219.4 1 N/A 24 28.0 2.5 26.0 6.0
4H-7N[I]:0 36 2 pEAG199R T293.2 2 1.00 60 0.1 0.1 0.0 0.0
4H-7N[I]:337[I] N/A N/A pEAG199B T265.1 2 0.06 48 106.5 6.9 109.5 35.0
4H-7N[D]:337[I] N/A N/A pEAG199D T267.6 1 N/A 24 32.1 4.2 28.0 13.0
4H-7N[I]:22:337[I] N/A N/A pEAG204E2 T322.10 2 0.22 48 61.94 4.6 61 19

Construct, a unique identifier of each repeat construct; %H, overall amount of homology (per cent), if applicable; #F, the number of triplet frames, if applicable; Plasmid, a unique identifier of the plasmid used to transform N. crassa strain FGSC#9720 to create homokaryotic Strain “A”; Replica crosses, the number of replica crosses analyzed for each repeat repeat construct; P-value, the lowest significance value of the Kolmogorov-Smirnov test obtained from pair-wise comparisons of replica crosses analyzed for each repeat construct; Sample size, the total number of spores analyzed for each repeat construct; Mean, the mean number of mutations (per spore) over the total sequenced region; SEM, standard error of the mean; Median, the median number of mutations (per spore) over the total sequenced region; MAD, the median absolute deviation from the median.