Table 1.
Antibodies used in the study (asterisks indicate antibodies used for flow cytometry)
Antibody | Manufacturer, catalog number | Species | Dilution |
---|---|---|---|
Primary antibodies | |||
AFP* | R&D, # MAB1368 | mouse anti-human/mouse IgG1 | 1:200 |
ALB* | R&D, # MAB1455 | mouse anti-human/mouse IgG2a | 1:200 |
CD29* | Millipore, # FCMAB269F | rat anti-mouse IgG2aκ | 2 μl per 106 cells |
CD49f* | Millipore, # MAB1378 | rat anti-human/mouse IgG2a | 1:10 |
CD45* | Millipore, # FCMAB126F | mouse anti-human/mouse IgG2bκ | 10 μl per 106 cells |
CD90* | Millipore, # CBL1500F | mouse anti-rat/mouse IgG1 | 10 μl per 106 cells |
CD133* | Millipore, # MAB4310X | rat anti- mouse IgG1κ | 1:200 |
CK8 | AbCam, # ab59400 | rabbit anti-human/rat/mouse IgG | 1:500 |
CK14 | Chemicon, # CBL197 | mouse anti-human IgG3 | 1:100 |
CK18 | Millipore, # MAB3234 | mouse anti-human/mouse IgG1 | 1:100 |
CK19* | AbCam, # ab15463-1 | rabbit anti-human/ mouse IgG | 1:100 |
CYP P450 1A1 | Millipore, # AB1258 | rabbit anti-human | 1:100 |
c-Kit* | Millipore, # CBL1360 | rat anti- mouse IgG2bκ | 1 μl per 106 cells |
c-Met* | Millipore, # MAB3729 | mouse anti-human/mouse IgG1 | 1:500 |
EpCAM* | Abcam, # ab32392 | rabbit anti-human/rat/mouse IgG | 1:500 |
Hnf-3β | Millipore, # 07-633 | rabbit anti-human/rat/mouse IgG | 1:200 |
Hnf-4α | Santa Cruz, # SC-8987 | rabbit anti-human/rat/mouse IgG | 1:200 |
INS | R&D, # MAB1417 | rat anti-human/bovine/mouse IgG2A | 1:200 |
Ki67 | Millipore, # MAB4190 | mouse anti-human IgG1 | 1:200 |
NGF | Millipore, # 04-1111 | rabbit anti-human/rat/mouse IgG | 1:200 |
Sca-1* | Millipore, # FCMAB224F | rat anti-mouse IgG2aκ | 2 μl per 106 cells |
Secondary antibodies | |||
Alexa Fluor® 488* | Invitrogen, # A-21206 | donkey anti-rabbit IgG (H + L) | 1:1000 |
Alexa Fluor® 546 | Invitrogen, # A-11035 | goat anti-rabbit IgG (H + L) | 1:1000 |
Alexa Fluor® 488* | Invitrogen, # A-11029 | goat anti-mouse IgG (H + L) | 1:1000 |
Alexa Fluor® 488* | Invitrogen, # A-11006 | goat anti-rat IgG (H + L) | 1:1000 |