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Chromatographic separation of charge-tagged monohydroxycholesterols. RIC for m/z 534.4054 corresponding to the [M]+ ion of monohydroxycholesterols (HC) from (A) Cyp46a1+/+, and (B) Cyp46a1−/−, mouse brain. (C) MRM of the transition 534→455→353 from Cyp46a1−/− mouse brain. (D) MRM of the transition 540→461→353 from 24R/S-hydroxycholesterol internal standard.
