Supplementary Figure 2.
Fascin is not required for PanIN formation. (A) Gene targeting strategy for generating fascin−/−, KRasG12D Pdx1-Cre (FKC). (B) Quantitation of spontaneous (4 months) PanIN formation in KC and FKC mice. Left: Dot plot of measurements of pancreas-to-body weight ratios. Middle: Box plot of amylase-positive area (lower quartile, median, and upper quartile are shown). Right 2 bar graphs: Relative number and percentage of PanINs of grade 1−3 per histopathologic section of pancreas. Data are shown as mean ± SEM (n = 6 mice per genotype). (C) Panels show tissue sections with spontaneous PanIN in FKC compared with KC mice; H&E (top), Alcian blue (middle), and dual immunohistochemistry (IHC) for CK19 (brown) and amylase (blue) (bottom). (D) Images and quantification of BrdU incorporation assay measured by IHC in duct cells (top) and acinar cells (bottom) in 4-month KC and FKC pancreas. Red arrows indicate BrdU-positive nuclei (n = 4 mice per genotype). (E) Panels show spontaneous PanIN formation in 6-week FKPC or KPC mice, with H&E (top) and dual IHC for CK19 (brown) and amylase (blue) (bottom). (F) Quantitation of spontaneous (6 weeks) PanIN formation in KPC and FKPC mice. Left: Box plot of amylase-positive area (lower quartile, median, and upper quartile are shown). Right 2 bar graphs: Number and percentage of PanINs of grades 1−3 per histopathologic section of pancreas. Data are shown as mean ± SEM (n = 5 mice per genotype). Scale bars in (C) and (E) = 100 μm and in (D) = 50 μm.