Supplementary Figure 4.

Tumor-intrinsic action of fascin. (A) Pancreata from Rosa26-LSL-tdRFP/+ Pdx1-Cre, fascin^−/− Rosa26-LSL-tdRFP/+ Pdx1-Cre and Pdx1-Cre mice were analyzed using an OV100 in vivo imaging system. Red fluorescent protein signaling is detected in whole pancreata from Rosa26-LSL-tdRFP/+ Pdx1-Cre and fascin^−/− Rosa26-LSL-tdRFP/+ Pdx1-Cre mice, but not in pancreata from mice only expressing Pdx1-Cre. (B) Representative images of immunohistochemical staining for Ki67, 2-hour BrdU pulse, phospho-histone H3 (PHH3), and cleaved capspase-3 (CC3). Quantification is shown in Figure 2E. Images (C) and quantifications (D) of infiltration of B cells (CD45R), T cells (CD3), macrophages (F4/80), and neutrophils (NIMP) into tumors. At least 16 medium-powered fields from 4 KPC and FKPC PDACs were analyzed. Data are shown as mean ± SEM. (E) Immunohistochemical visualization of platelet endothelial cell adhesion molecule (CD31) in tumors in KPC and FKPC mice. (F) Quantification of (D), no significant difference in vascularization. Data are shown as mean ± SEM (n = 4 mice from each genotype). Scale bars = 100 μm.