Supplementary Figure 6.

Slug is an important regulator of fascin expression. (A) Immunofluorescence microscopy analysis of 070669 PDAC cells transduced with vector (control), Flag-slug, Flag-snail, and twist and stained for the proteins as indicated. Slug, snail, and twist overexpressing cells are indicated by yellow arrows. (B) Western blots (left) and quantitation (right) of proteins as indicated with 070669 PDAC cells treated with slug siRNA. Data are shown as mean ± SEM. ^∗∗P < .01 by Student t test. Loading control: α-actinin. (C) Western blot analysis of proteins as indicated with 070669 PDAC cells treated with Zeb1 (top left), Zeb2 (top right), E-cadherin (bottom left), and fascin (bottom right) siRNA. Loading controls: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α-actinin, as indicated. (D) Phase contrast images of 061843 PDAC cells transduced with vector (control), Flag-slug, Flag-snail, and twist as indicated. (E) Western blot analysis with control, Flag-slug, Flag-snail, and twist expressing 061843 PDAC cells for proteins as indicated. Loading control: α-actinin. (F) Western blot analysis with control, Flag-slug, Flag-snail expressing PANC-1 (left) and HT29 (right) human pancreatic and colon cancer cells for proteins as indicated. Loading control: α-actinin. Scale bars in (A) = 10 μm and in (D) = 50 μm.