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. 2014 May;146(5):1386–1396.e17. doi: 10.1053/j.gastro.2014.01.046

Figure 5.

Figure 5

Fascin is a slug target gene. (A) Schematic showing the potential E-box element in intron 1 of the fascin gene and regions targeted by 3 primer pairs (#1−3, red lines). Primer pair #1 targets the putative E-box, and primer pair and #2 and #3 target downstream regions. The number in parentheses indicates distance downstream of transcription start site. (B) Chromatin from 070669 PDAC cells expressing Flag-slug was immunoprecipitated using Flag antibody and polymerase chain reaction was performed on the chromatin Immunoprecipitation product using 3 primer pairs. Primers for an E-cadherin promoter E-box element were used as positive control (mean ± SEM). ∗∗P < .01 by Student t test. (C) Top: the putative E-box on the mouse fascin gene and mutations. Bottom: Relative luciferase activities of 070669 PDAC cells transfected as indicated. n = 3 experiments (mean ± SEM). ∗∗P < .01; ∗P < .05 by Student t test.