Table 1.
Primers | Sequences | Annealing temp (°C) | Size (bp) |
MT1M | |||
U | 5'-TTGAAAATGGTGGGGTGA-3' | 54 | 163 |
5'-AAACTATACACCAAATAATACACAATATCC-3' | |||
M | 5'-GACGTTCGCGACGTTAAG-3' | 54 | 124 |
5'-ACGCCGAATAATACGCAAT-3' | |||
MT1G | |||
U | 5'-GGGGTTGTTTTGTGGTGTGTG-3' | 59 | 135 |
5'-AAACACCCCACCCCACCCTT-3' | |||
M | 5'-TTCGCGAGTCGGTGCGAAAG-3' | 50 | 96 |
5'-CCGCGATCCCGACCTAAACT-3' |